This study was conducted to identify mycobacteria in cattle and buffalo by using conventional method of Tuberculin and then by ELISA using serum samples in randomly selected animals. Positive sample of ELISA were further run on PCR for confirmation. Furthermore, along with the checking of its comparative efficacy, the disease prevalence in cattle and buffalo on the basis of associated risk factors including the species, age, sex and management, in vicinity of Lahore, Pakistan were investigated. One hundred and ninety-two clinically suspected animals of different breeds, ages (2-8 years) and weights for detection of tuberculosis, were selected randomly from four organized farm of Lahore district. The animals were divided into two main experimental groups designated as group B (buffalos) and group C (cows). Each main group was further sub-divided into 3 sub-groups B1, B2, B3 and C1, C2, C3 with age group of below 4y, 4-6y and over 6y, respectively. On the basis of tuberculin test number of positive cases of bovine tuberculosis in buffalo were 7.29 % while in cattle were 11.46% out of total of 96 animals of each.
Tuberculin positive cases were further tested with PCR and ELISA. Number of positive cases of buffalo and cattle were 85.71% and 90.91%, respectively when tested with PCR. The expected size of the amplified DNA fragment was 317 bp. On the basis of diagnosis with ELISA-IFN-γ assay, number of positive cases in buffalo and cattle were 71.43%% and 72.73%, respectively.
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