In-vitro antibacterial activity of aqueous, alcoholic, and chloroform extracts of leaves of Rauvolfia tetraphylla L. and Jatropha curcas L. against various bacteria along with their photochemical analysis was studied. The antibacterial activities of different extracts were evaluated by disc diffusion assay with standard antibacterial drugs as a positive control in the present study. Methanol extract of both plants have shown good and comparable antimicrobial activity against most gram positive and gram negative bacteria tested which was in the range of except against S. typhimurim and E. Coli . Chloroform extract of Rauvolfia tetraphylla was also effective against majority of bacteria (12.00 0.58 to 14.67 0.88 mm) but Chloroform extract of Jatropha curcas had activity against S. aureus, K. pneumonia and P. vulgaris only with zone of inhibition in the range of (12.67 1.20 to 14.33 2.03 mm). Water extracts of both the plants did not show any antibacterial activity. Phytochemical screening revealed the presence of alkaloids, tannins, flavonoids, saponins, diterpenes, triterpenes, and carbohydrates in the methanol extracts of both the plants. The potency of the crude extracts of Rauvolfia tetraphylla and Jatropha curcas to inhibit the growth of bacteria is an indication of its antimicrobial potential which may be useful to explore its efficacy against bacterial infections. 1377753231.php