A Polymerase Chain Reaction � Restriction Fragment Length Polymorphism test was performed on DNA samples extracted from blood samples of 98 unrelated Nagpuri buffaloes to detect genotype frequency and allelic frequency of kappa casein and beta-lactoglobulin gene responsible for milk production traits. The restriction digestion analysis of 350 bp PCR product of kappa casein indicates the presence of only one type of restriction pattern, BB-genotype of two fragments of 266 and 84 bp. The restriction digestion analysis of the 247 bp PCR product of beta-lactoglobulin surprisingly indicated single pattern, AB-genotype of three fragments of 148, 99, 74 bp (heterogygous). The heterozygous pattern of all the 98 buffaloes indicates that random samples were coincidentally collected from such animals. The results indicated 1.0 allelic frequency of B-allele for kappa-casein whereas 0.5 allelic frequencies were observed for each A and B alleles of Beta-lactoglobulin. Kappa-casein and beta-lactoglobulin are useful markers for milk production traits on which animals can be evaluated and selected for future breeding programmes. 1317874745.php