Collection techniques and corpus luteum effects on quality and quantity of goat follicular oocytes for in vitro studies
A.N.M.I. Rahman*, L. Asad, M.M. Hossain
Date : 2016-07-21 Volume : 8

The present study was undertaken to assess the relative effects of collection techniques and presence or absence of corpus luteum (CL) on recovery efficiency and oocyte quality for in vitro studies of goat oocytes. Cumulus-oocyte-complexes (COCs) were collected from slaughter house goat ovaries by aspiration, puncture and slicing methods. Following collection; oocyte qualities were classified into normal and abnormal group on the basis of cumulus attachment. The mean number of oocytes recovered per ovary was significantly (P<0.05) higher in slicing (5.34±0.35) than that of puncture (4.15±0.23) and aspiration (3.56±0.15) techniques. The normal grade oocyte per ovary was significantly (P<0.05) higher in aspiration (54.78%) and in puncture (54.70%) method than slicing (30.71%). Furthermore, the oocyte recovery was significantly lower (P<0.05) in CL containing ovaries (2.02±0.23) than that of ovaries without CL (4.92±0.27). It was observed that significantly (p<0.05) higher number of normal quality COCs per ovary were obtained from without CL ovaries compared to ovaries having CL with the mean of (2.47±0.12) and (0.98±0.14) respectively. Thus, it can be concluded that, slicing technique recovered more oocytes per ovary and without CL ovaries is a good source of normal grade oocytes for in vitro production of goat embryos. 1454132647.php