The aim of this study was to compare the effects of using different levels of royal jelly in semen extender on vital characteristics parameters of goat sperm after freezing. Semen was collected from three adult bucks twice a week and samples pooled together. Sperm samples (seven repeats) containing royal jelly in five levels (0,0.5, 1, 1.5 and 2%) had been frozen. After freezing semen samples were thawed and examined in terms of motility, survival and integrity of the plasma membrane, normal acrosome and DNA integrity . Average total motility, livability, plasma membrane integrity and DNA % at 1% royal jelly were higher than other treatment and control groups (P<0.05). Therefore, the use of royal jelly in goat semen diluent can improve some sperm parameters after freezing process. 1482317240.php