Colibacillosis occurs as an acute fatal septicaemic disease of young buffalo calves and is seen worldwide. The main determinant of the disease is decreased immune status of the young one along with improper management and stress factors, which also enhances the growth of opportunistic bacteria. The colisepticemic form of colibacillosis usually associated with an E. coli bacteraemia and results in the rapid death of the calf. The present paper highlighted the incidence and pathology of E. coli infection on liver of buffalo calves. The incidence of colibacillosis affecting liver was recorded as 11.52 per cent. Grossly, the affected liver showed focal areas of haemorrhages and presence of grayish, white, necrotic foci. Microscopically, liver sections showed varying degree of degenerative changes viz. fatty changes and coagulative necrosis, hyperplasia and proliferation of bile duct and dilated, blood filled hepatic sinusoids along with congested central vein. Some sections revealed hepatitis due to focal areas of infiltrating cells, predominantly neutrophils and few macrophages. 1362553142.php