Levofloxacin is third generation fluoroquinone having broad-spectrum antibacterial activity. Ketoprofen is non-steroidal anti-inflammatory drug commonly used in veterinary medicine. The present study was planned to determine blood biochemical profile following daily intravenous administration of levofloxacin (3 mg/kg) along with intramuscular administration of ketoprofen (3 mg/kg) for 5 days in sheep. Blood samples were collected daily for 5 days before and after the initiation of drugs administration and proceeded for determination of haematological (Hb, PCV, TEC, TLC and DLC) and biochemical parameters (AST, ALT, LDH, ACP, AKP, serum creatinine, serum bilirubine, BUN, serum total protein, serum albumin, serum globulin and blood glucose). Hematological and biochemical profile before and after the treatment revealed that levofloxacin did not have any significant impact on major blood parameters except BUN which are altered in disease or pathological conditions. Thus, the drug may be useful in combination of ketoprofen to treat bacterial diseases accompanied by febrile condition in sheep. 1351160210.php